For whole-genome sequencing, the combination of short inserts and longer reads allow characterization of any genome. For de novo whole-genome sequencing, the unparalleled raw read accuracy of Illumina next-generation sequencing (NGS) technology provides high quality, long contig assemblies.
Sample Preparation for Whole-Genome Sequencing
The Illumina whole-genome sequencing workflow starts with the generation of sequencer-ready DNA fragment libraries from genomic DNA. Illumina offers two basic options:
- TruSeq DNA family: Recommended for preparing human or other complex whole genomes. This includes the PCR-Free and Nano kits.
- Nextera family: Recommended for rapid genome preparation. This includes the Nextera XT Sample Prep Kit, suitable for small genomes such as bacteria and viruses.
Phased Sequencing Complements Whole-Genome Sequencing
Phased sequencing complements human whole-genome sequencing by providing distinct gene information from homologous chromosomes, supporting haplotyping and allele-specific solutions. Learn more about:
Whole-Genome Sequencers and Data Analysis Solutions
Illumina offers a broad range of instruments and informatics solutions that enable whole genome sequencing of any genome, from small microbial genomes to large complex genomes.