Whole-Genome Sequencing

Overview

For whole-genome sequencing, the combination of short inserts and longer reads allow characterization of any genome. For de novo whole-genome sequencing, the unparalleled raw read accuracy of Illumina next-generation sequencing (NGS) technology provides high quality, long contig assemblies.

Sample Preparation for Whole-Genome Sequencing

The Illumina whole-genome sequencing workflow starts with the generation of sequencer-ready DNA fragment libraries from genomic DNA. Illumina offers two basic options:

  • TruSeq DNA family: Recommended for preparing human or other complex whole genomes. This includes the PCR-Free and Nano kits.
  • Nextera family: Recommended for rapid genome preparation. This includes the Nextera XT Sample Prep Kit, suitable for small genomes such as bacteria and viruses.

Phased Sequencing Complements Whole-Genome Sequencing

Phased sequencing complements human whole-genome sequencing by providing distinct gene information from homologous chromosomes, supporting haplotyping and allele-specific solutions. Learn more about: 

Whole-Genome Sequencers and Data Analysis Solutions

Illumina offers a broad range of instruments and informatics solutions that enable whole genome sequencing of any genome, from small microbial genomes to large complex genomes.

Related Solutions

Desktop Genome Sequencer

 

The NextSeq 500 System can sequence a whole human genome at high coverage (30×) in one run.
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Library Prep Kit Selector

Product Selector

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Whole-Genome Sequencing of Complex Microbial Mixtures

Shotgun metagenomic sequencing allows researchers to sequence all organisms present in a given sample, including unculturable microbes.

Automation Partners

Reduce hands-on time and minimize errors with sequencing sample prep automation solutions.

Featured Products & Services

TruSeq DNA PCR-Free Sample Preparation Kit
TruSeq DNA PCR-Free Sample Preparation Kit

Shortened sample prep workflow for whole genome sequencing applications.

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TruSeq Nano DNA Sample Prep Kit
TruSeq Nano DNA Sample Prep Kit

Enables efficient interrogation of samples with limited available DNA.

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Nextera XT Sample Prep Kit
Nextera XT Sample Prep Kit

Enables the fastest and easiest whole-genome library preparation method for sequencing small genomes, amplicons, and plasmids.

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BaseSpace

The BaseSpace NGS Data Analysis environment offers simple analysis and sharing of whole-genome sequencing data produced on Illumina sequencers.

The Illumina Genome Network offers human whole-genome sequencing services.