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For whole-genome sequencing, the combination of short inserts and longer reads allow characterization of any genome. For de novo whole-genome sequencing, the unparalleled raw read accuracy of Illumina next-generation sequencing (NGS) technology provides high quality, long contig assemblies.
The Illumina whole-genome sequencing workflow starts with the generation of sequencer-ready DNA fragment libraries from genomic DNA. Illumina offers two basic options:
Phased sequencing complements human whole-genome sequencing by providing distinct gene information from homologous chromosomes, supporting haplotyping and allele-specific solutions. It enables researchers to distinguish between maternally and paternally inherited alleles.
Illumina offers a broad range of instruments and informatics solutions that enable whole genome sequencing of any genome, from small microbial genomes to large complex genomes.
The NextSeq 500 System can sequence a whole human genome at high coverage (30×) in one run.
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Shotgun metagenomic sequencing allows researchers to sequence all organisms present in a given sample, including unculturable microbes.
Reduce hands-on time and minimize errors with sequencing sample prep automation solutions.